abstract：:The dipeptide glycyl-l-phenylalanine 2-naphthylamide (GPN) is widely used to perturb lysosomes because its cleavage by the lysosomal enzyme cathepsin C is proposed to rupture lysosomal membranes. We show that GPN evokes a sustained increase in lysosomal pH (pHly), and transient increases in cytosolic pH (pHcyt) and Ca...

journal_title：Journal of cell science

authors： Atakpa P,van Marrewijk LM,Apta-Smith M,Chakraborty S,Taylor CW

更新日期：2019-02-01 00:00:00

abstract：:Compartmentalization of membrane transport and signaling processes is of pivotal importance to eukaryotic cell function. While plasma membrane compartmentalization and dynamics are well known to depend on the scaffolding function of septin GTPases, the roles of septins at intracellular membranes have remained largely ...

journal_title：Journal of cell science

authors： Song K,Gras C,Capin G,Gimber N,Lehmann M,Mohd S,Puchkov D,Rödiger M,Wilhelmi I,Daumke O,Schmoranzer J,Schürmann A,Krauss M

更新日期：2019-02-01 00:00:00

abstract：:In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection. Knockdown of PLIN2 did not affect LD homeostasis, likely due to compensation by PLIN3, bu...

journal_title：Journal of cell science

authors： Lassen S,Grüttner C,Nguyen-Dinh V,Herker E

更新日期：2019-01-09 00:00:00

abstract：:Junction dynamics of endothelial cells are based on the integration of signal transduction, cytoskeletal remodeling and contraction, which are necessary for the formation and maintenance of monolayer integrity, but also enable repair and regeneration. The VE-cadherin-catenin complex forms the molecular basis of the ad...

journal_title：Journal of cell science

authors： Cao J,Schnittler H

更新日期：2019-01-03 00:00:00

abstract：:The Birt-Hogg-Dubé (BHD) syndrome related protein FLCN has recently been implicated in the vesicular trafficking processes by interacting with several Rab family GTPases. In the previous studies, we have shown that FLCN could inhibit the binding of overexpressed PAT1, which is a membrane-bound amino acid transporter, ...

journal_title：Journal of cell science

authors： Zhao L,Ji X,Zhang X,Li L,Jin Y,Liu W

更新日期：2018-12-19 00:00:00

abstract：:How vesicle trafficking components actively contribute to regulation of paracrine signaling is unclear. We genetically uncovered a requirement for α-soluble NSF attachment protein (α-Snap) in the activation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway during Drosophila egg de...

journal_title：Journal of cell science

authors： Saadin A,Starz-Gaiano M

更新日期：2018-11-30 00:00:00

abstract：:In fibroblasts, platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) stimulate the formation of actin-rich, circular dorsal ruffles (CDRs) and phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of Akt. To test the hypothesis that CDRs increase synthesis of phosphorylated Akt1 (pAkt), we ...

journal_title：Journal of cell science

authors： Yoshida S,Pacitto R,Sesi C,Kotula L,Swanson JA

更新日期：2018-11-16 00:00:00

abstract：:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...

journal_title：Journal of cell science

authors： Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg A

更新日期：2018-11-05 00:00:00

abstract：:Gene expression of malaria parasites is mediated by the apicomplexan Apetala2 (ApiAP2) transcription factor family. Different ApiAP2s control gene expression at distinct stages in the complex life cycle of the parasite, ensuring timely expression of stage-specific genes. ApiAP2s recognize short cis-regulatory elements...

journal_title：Journal of cell science

authors： Klug D,Kehrer J,Frischknecht F,Singer M

更新日期：2018-10-22 00:00:00

abstract：:The degradation of misfolded proteins is essential for cellular homeostasis. Misfolded proteins are normally degraded by the ubiquitin-proteasome system (UPS), and selective autophagy serves as a backup mechanism when the UPS is overloaded. Selective autophagy mediates the degradation of harmful material by its seques...

journal_title：Journal of cell science

authors： Danieli A,Martens S

更新日期：2018-10-04 00:00:00

abstract：:Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing mela...

journal_title：Journal of cell science

authors： Nag S,Rani S,Mahanty S,Bissig C,Arora P,Azevedo C,Saiardi A,van der Sluijs P,Delevoye C,van Niel G,Raposo G,Setty SRG

更新日期：2018-09-20 00:00:00

abstract：:Cancer cells thrive when challenged with proteotoxic stress by inducing components of the protein folding, proteasome, autophagy and unfolded protein response (UPR) pathways. Consequently, specific molecular chaperones have been validated as targets for anti-cancer therapies. For example, inhibition of Hsp70 family pr...

journal_title：Journal of cell science

authors： Sannino S,Guerriero CJ,Sabnis AJ,Stolz DB,Wallace CT,Wipf P,Watkins SC,Bivona TG,Brodsky JL

更新日期：2018-09-05 00:00:00

abstract：:It is generally accepted that global translation varies during the cell cycle and is low during mitosis. However, addressing this issue is challenging because it involves cell synchronization, which evokes stress responses that, in turn, affect translation rates. Here, we have used two approaches to measure global tra...

journal_title：Journal of cell science

authors： Stonyte V,Boye E,Grallert B

更新日期：2018-09-03 00:00:00

abstract：:Rab GTPases are key regulators of intracellular trafficking, and cycle between a GTP-bound active state and a GDP-bound inactive state. This cycle is regulated by guanine-nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Several efforts have been made in connecting the correct GEFs and GAPs to ...

journal_title：Journal of cell science

authors： Borg Distefano M,Hofstad Haugen L,Wang Y,Perdreau-Dahl H,Kjos I,Jia D,Morth JP,Neefjes J,Bakke O,Progida C

更新日期：2018-09-03 00:00:00

abstract：:Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...

journal_title：Journal of cell science

authors： Lisowska J,Rödel CJ,Manet S,Miroshnikova YA,Boyault C,Planus E,De Mets R,Lee HH,Destaing O,Mertani H,Boulday G,Tournier-Lasserve E,Balland M,Abdelilah-Seyfried S,Albiges-Rizo C,Faurobert E

更新日期：2018-08-13 00:00:00

abstract：:Although the kinase CHK1 is a key player in the DNA damage response (DDR), several studies have recently provided evidence of DDR-independent roles of CHK1, in particular following phosphorylation of its S280 residue. Here, we demonstrate that CHK1 S280 phosphorylation is cell cycle-dependent and peaks during mitosis....

journal_title：Journal of cell science

authors： Adam K,Cartel M,Lambert M,David L,Yuan L,Besson A,Mayeux P,Manenti S,Didier C

更新日期：2018-08-10 00:00:00

abstract：:Gap junctions (GJs) assembled from connexin (Cx) proteins allow direct cell-cell communication. While phosphorylation is known to regulate multiple GJ functions, much less is known about the role of ubiquitin in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show ...

journal_title：Journal of cell science

authors： Kells-Andrews RM,Margraf RA,Fisher CG,Falk MM

更新日期：2018-08-09 00:00:00

abstract：:In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome to the host cell cytoplasm and to avoid degradation, viruses have to escape this endoso...

journal_title：Journal of cell science

authors： Staring J,Raaben M,Brummelkamp TR

更新日期：2018-08-03 00:00:00

abstract：:The activation of phospholipase C (PLC) is a conserved mechanism of receptor-activated cell signaling at the plasma membrane. PLC hydrolyzes the minor membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], and continued signaling requires the resynthesis and availability of PI(4,5)P2 at the plasma membrane....

journal_title：Journal of cell science

authors： Balakrishnan SS,Basu U,Shinde D,Thakur R,Jaiswal M,Raghu P

更新日期：2018-08-03 00:00:00

abstract：:During growth, homeostasis and regeneration, stem cells are exposed to different energy demands. Here, we characterise the metabolic pathways that mediate the commitment and differentiation of mouse skeletal muscle stem cells, and how their modulation can influence the cell state. We show that quiescent satellite stem...

journal_title：Journal of cell science

authors： Pala F,Di Girolamo D,Mella S,Yennek S,Chatre L,Ricchetti M,Tajbakhsh S

更新日期：2018-07-27 00:00:00

abstract：:Annexins are cytosolic phospholipid-binding proteins that can be found on the outer leaflet of the plasma membrane. The extracellular functions of annexin include modulating fibrinolysis activity and cell migration. Despite having well-described extracellular functions, the mechanism of annexin transport from the cyto...

journal_title：Journal of cell science

authors： Stewart SE,Ashkenazi A,Williamson A,Rubinsztein DC,Moreau K

更新日期：2018-07-19 00:00:00

abstract：:The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal de...

journal_title：Journal of cell science

authors： Ferrari ME,Bernis ME,McLeod F,Podpolny M,Coullery RP,Casadei IM,Salinas PC,Rosso SB

更新日期：2018-07-09 00:00:00

abstract：:Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...

journal_title：Journal of cell science

authors： Ben-Shimon L,Paul VD,David-Kadoch G,Volpe M,Stümpfig M,Bill E,Mühlenhoff U,Lill R,Ben-Aroya S

更新日期：2018-06-27 00:00:00

abstract：:Most secretory proteins travel through a well-documented conventional secretion pathway involving the endoplasmic reticulum (ER) and the Golgi complex. However, recently, it has been shown that a significant number of proteins reach the plasma membrane or extracellular space via unconventional routes. Unconventional p...

journal_title：Journal of cell science

authors： Kim J,Gee HY,Lee MG

更新日期：2018-06-25 00:00:00

abstract：:Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension. Despite their importance, how hemidesmosomes are regulated at the post-transcriptional level is poorly understood. Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to the...

journal_title：Journal of cell science

authors： Fu R,Zhu Y,Jiang X,Li Y,Zhu M,Dong M,Huang Z,Wang C,Labouesse M,Zhang H

更新日期：2018-06-11 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is the most common monogenic human disease, with around 12.5 million people affected worldwide. ADPKD results from mutations in either PKD1 or PKD2, which encode the atypical G-protein coupled receptor polycystin-1 (PC1) and the transient receptor potential channel ...

journal_title：Journal of cell science

authors： Tilley FC,Gallon M,Luo C,Danson CM,Zhou J,Cullen PJ

更新日期：2018-06-06 00:00:00

abstract：:Correct spindle orientation is achieved through signaling pathways that provide a molecular link between the cell cortex and spindle microtubules in an F-actin-dependent manner. A conserved cortical protein complex, composed of LGN (also known as GPSM2), NuMA (also known as NUMA1) and dynein-dynactin, plays a key role...

journal_title：Journal of cell science

authors： Kschonsak YT,Hoffmann I

更新日期：2018-05-21 00:00:00

abstract：:The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...

journal_title：Journal of cell science

authors： Jojic B,Amodeo S,Bregy I,Ochsenreiter T

更新日期：2018-05-10 00:00:00

abstract：:Heat shock transcription factor 1 (HSF1) regulates the expression of a wide array of genes, controls the expression of heat shock proteins (HSPs) as well as cell growth. Although acute depletion of HSF1 induces cellular senescence, the underlying mechanisms are poorly understood. Here, we report that HSF1 depletion-in...

journal_title：Journal of cell science

authors： Oda T,Sekimoto T,Kurashima K,Fujimoto M,Nakai A,Yamashita T

更新日期：2018-05-08 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves changes in their subcellular localization, indicating an important role for these spatio-temporal dynamics in signal transmission. A striking model illu...

journal_title：Journal of cell science

authors： Serrano A,Illgen J,Brandt U,Thieme N,Letz A,Lichius A,Read ND,Fleißner A

更新日期：2018-05-04 00:00:00

abstract：:Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of the non-muscle actin...

journal_title：Journal of cell science

authors： Hatano T,Alioto S,Roscioli E,Palani S,Clarke ST,Kamnev A,Hernandez-Fernaud JR,Sivashanmugam L,Chapa-Y-Lazo B,Jones AME,Robinson RC,Sampath K,Mishima M,McAinsh AD,Goode BL,Balasubramanian MK

更新日期：2018-04-23 00:00:00

abstract：:Localization and translation of mRNAs within different subcellular domains provides an important mechanism to spatially and temporally introduce new proteins in polarized cells. Neurons make use of this localized protein synthesis during initial growth, regeneration and functional maintenance of their axons. Although ...

journal_title：Journal of cell science

authors： Sahoo PK,Smith DS,Perrone-Bizzozero N,Twiss JL

更新日期：2018-04-13 00:00:00

abstract：:During the prometaphase stage of mitosis, the cell builds a bipolar spindle of microtubules that mechanically segregates sister chromatids between two daughter cells in anaphase. The spindle assembly checkpoint (SAC) is a quality control mechanism that monitors proper attachment of microtubules to chromosome kinetocho...

journal_title：Journal of cell science

authors： Courtheoux T,Diallo A,Damodaran AP,Reboutier D,Watrin E,Prigent C

更新日期：2018-04-12 00:00:00

abstract：:Microscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation toleranc...

journal_title：Journal of cell science

authors： Cardon ZG,Peredo EL,Dohnalkova AC,Gershone HL,Bezanilla M

更新日期：2018-04-10 00:00:00

abstract：:The cellular microenvironment, characterized by an extracellular matrix (ECM), played an essential role in the transition from unicellularity to multicellularity in animals (metazoans), and in the subsequent evolution of diverse animal tissues and organs. A major ECM component are members of the collagen superfamily -...

journal_title：Journal of cell science

authors： Fidler AL,Boudko SP,Rokas A,Hudson BG

更新日期：2018-04-09 00:00:00

abstract：:Sexual dimorphism has been used to describe morphological differences between the sexes, but can be extended to any biologically related process that varies between males and females. The synaptonemal complex (SC) is a tripartite structure that connects homologous chromosomes in meiosis. Here, aided by super-resolutio...

journal_title：Journal of cell science

authors： Agostinho A,Kouznetsova A,Hernández-Hernández A,Bernhem K,Blom H,Brismar H,Höög C

更新日期：2018-03-06 00:00:00

abstract：:Unconventional myosin VIIA (Myo7a) is an actin-based motor molecule that normally functions in the cochlear hair cells of the inner ear. Mutations of MYO7A/Myo7a have been implicated in inherited deafness in both humans and mice. However, there is limited information about the functions of Myo7a outside of the special...

journal_title：Journal of cell science

authors： Liu Y,Wei X,Guan L,Xu S,Yuan Y,Lv D,He X,Zhan J,Kong Y,Guo J,Zhang H

更新日期：2018-02-22 00:00:00

abstract：:In the developing Drosophila eye, the centrioles of the differentiating retinal cells are not surrounded by the microtubule-nucleating γ-tubulin, suggesting that they are unable to organize functional microtubule-organizing centers. Consistent with this idea, Cnn and Spd-2, which are involved in γ-tubulin recruitment,...

journal_title：Journal of cell science

authors： Riparbelli MG,Persico V,Gottardo M,Callaini G

更新日期：2018-02-22 00:00:00

abstract：:Stress can be temporary or chronic, and mild or acute. Depending on its extent and severity, cells either alter their metabolism, and adopt a new state, or die. Fluctuations in environmental conditions occur frequently, and such stress disturbs cellular homeostasis, but in general, stresses are reversible and last onl...

journal_title：Journal of cell science

authors： Kucherenko MM,Shcherbata HR

更新日期：2018-02-14 00:00:00

abstract：:Skeletal muscle is primarily composed of large myofibers containing thousands of post-mitotic nuclei distributed throughout a common cytoplasm. Protein production and localization in specialized myofiber regions is crucial for muscle function. Myonuclei differ in transcriptional activity and protein accumulation, but ...

journal_title：Journal of cell science

authors： Cutler AA,Jackson JB,Corbett AH,Pavlath GK

更新日期：2018-02-05 00:00:00